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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG <t>analysis</t> on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular <t>heart</t> <t>rate</t> was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.
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Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG analysis on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular heart rate was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.

Journal: Scientific Reports

Article Title: Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model

doi: 10.1038/s41598-019-47455-3

Figure Lengend Snippet: Functional abnormality in electrocardiogram (EKG) in an oral infection mouse model. The 3-day-old NOD/SCID mice were infected orally with 10 7 pfu of EV71-F6 (n = 7), EV71-F23 (n = 4) or normal saline. Infected mice were examined by EKG analysis on day 16 post-infection. ( A ) A representative configuration of a typical mouse electrocardiogram is derived from an average of four consecutive heartbeats of an uninfected littermate. P wave: the electrical manifestation of spread of atrial excitation; QRS complex: the electrical manifestation of spread of ventricular excitation; T wave: repolarization of the ventricles. ( B ) EKG in one-second recording showed a consistent split of P wave in F6-Mouse-1, PR segment depression in F6-Mouse-1, -3, -4 and -5, and ST elevation in F6-Mouse-3, and mild ST elevation in F6-Mouse-6. In two-second recording, irregular heart rate was evident in F6-mouse-7. ( C ) RR intervals showed irregular heart rate in F6-Mouse-2 and -7. ( D ) EKG showed irregular heart rate in mouse F23-Mouse-1 and -4, shortened or no R amplitude in mouse F23-Mouse-2, apparent ST elevation in F23-Mouse-2 and -3, and nearly abolished P wave in mouse F23-Mouse-4. ( E ) A Waterflow Plot resolved split P peaks and R amplitude in F23-mouse-2.

Article Snippet: A continuous steady 5-min EKG recording was then selected randomly for the waveform and heart rate variant analysis (LabChart, ADInstruments) .

Techniques: Functional Assay, Infection, Saline, Derivative Assay